Cell Tissue Res 269:65–77Ĭhen Y, Veenstra JA, Davis NT, Hagedorn HH (1994) A comparative study of leucokinin-immunoreactive neurons in insects. Cell Tissue Res 269:459–471Ĭantera R, Hansson BS, Hallberg E, Nässel DR (1992) Postembryonic development of leucokinin I-immunoreactive neurons innervating a neurohemal organ in the turnip moth Agrotis segetum. Biochem Biophys Res Commun 181:927–932Ĭantera R, Nässel DR (1992) Segmental peptidergic innervation of abdominal targets in larval and adult dipteran insects revealed with an antiserum against leucokinin I. Ann Entomol Soc Am 69:365–373īlackburn MB, Kingan TG, Bodnar W, Shabanowitz J, Hunt D, Kempe T, Wagner RM, Raina AK, Schnee ME, Ma MC (1991) Isolation and identification of a new diuretic peptide from the tobacco hornworm, Manduca sexta. J Exp Biol 178:231–243īell RA, Joachim FA (1976) Techniques for rearing laboratory colonies of tobacco hornworms and pink bollworms. This loss appears to be related to the change from the larval to adult pattern of diuresis.Īudsley N, Coast GM, Schooley DA (1993) The effects of Manduca sexta diuretic hormone on fluid transport by the Malpighian tubules and cryptonephretic complex of Manduca sexta. The immunoreactivity of these cells is lost as the cryptonephridium is eliminated during metamorphosis. A median pair of leucokinin-immunoreactive, and a lateral pair of diuretic hormone-immunoreactive neurons in the larval terminal abdominal ganglion project to neurohemal release sites within the cryptonephridium.

Diuretic hormone and leucokinin immunoreactivity are co-localized in the lateral neurosecretory cells and their neurohemal projections. sexta diuretic hormone were also investigated. Relationships of these neurons to cells that are immunoreactive to a M. The immunoreactivity and size of these lateral cells greatly increases in the pharate adult, and this change appears to be related to a period of intensive diuresis occurring a few days before adult eclosion. Bilateral pairs of neurosecretory cells in abdominal ganglia 3–7 of larvae and adults are immunoreactive these cells project via the ipsilateral ventral nerves to the neurohemal transverse nerves.

Immunostaining was done at various stages of development, using an antiserum to leucokinin IV. Because leucokinins stimulate diuresis in some insects, we wished to identify the neurosecretory cells in Manduca sexta that might be a source of leucokinin-like neurohormones.

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